Granzyme A was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Human Granzyme A Antigen Affinity-purified Polyclonal Antibody at 15ug/ml for 3h at room temperature. Cells were stained using the NorthernLightsTM 557-conjugated Goat IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.